Molecular Life Science - Blockpraktikum II (Modul

Crosstalk between and intracellular signalling/gene regulation in neurons, astrocytes and brain endothelial cells (Jöhren Methods: Primary cell culture/co-cultures, gene transfection, siRNA knockdown, qPCR, reporter gene assays Kompetenzen: S4, Z1, Z2, Z3

Generation of new AAV-Vektor systems to manipulate neuronal cells (endothelia, neuron, astrocytes) in living mice (Müller-Fielitz, Dogbevia Methods: gene transfer via AAV-Systems, production of AAVs, stereotactic injections, cloning, qPCR, immunostaining Kompetenzen: S4, Z1, Z3, Z4, Z5

2-Photon microscopy of the brain microvasculature (Wenzel) Methods: chronic/acute cranial window in mice, physiological and pharmacolopgical effects on blood flow and morphology (e.g. calcium imaging; neurovascular coupling, different genotypes); image analysis Kompetenzen: S5, Z2; Z4, Z5

Functional and morphological analysis of conditional brain-endothelial knock-out mice (Ridder) Methods: Primary cell culture, Western Blot, immunofluorescent staining and microscopy, animal experiments Kompetenzen: Z1, S2, Z4, Z5